Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage dewar. Cells are viable for at least 2 years when stored under these conditions.
Proliferating Normal Human Bronchial Epithelial Cells- P1 (NhBE-P1) are isolated, initially as NhBE-P0, from the bronchus at the bifurcation point to the fourth generation of bronchi. The original cell composition consisted of a mix of differentiated ciliated, non-ciliated, goblet, and basal cells. After culturing the mixed NhBE-P0 in BEGM, CRC or Dual Smad culture conditions, the result is a homogenous population of basal cells, or NhBE-P1.
Proliferating NhBE-P1 cells have been characterized by morphological assessment by light microscopy. The NhBE-P1 homogenous basal cell population was then further QC tested after differentiating the NhBE-P1 cells (using Novabiosis ALI media) Proliferating Normal Human Bronchial Epithelial Cells-P1 (NhBE-P1) into ciliated, non-ciliated and goblet cells. These differentiated cells were characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).
The NhBE-P1 cells are guaranteed to have minimum population doublings of at least 20 (from P1 to P3) following the instructions and conditions provided by Novabiosis.
Proliferating NhBE-P1 are for research use only and not approved for human or animal use, nor for in vitro diagnostic testing.