Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Normal Human Glomerular Endothelial Cells (NhGEC) are specialized microvascular cells that make up the innermost layer of the glomerular filtration barrier. They are separated from podocytes by a thin layer of glomerular basement membrane. These cells exhibit cobblestone morphology at confluence and present fenestrations specific of this cell type.
The NhGEC are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhGEC are isolated by CD31 positive selection as a pure population.
The NhGEC express factor VIII-related antigen, angiotensin converting enzyme activity, and endocytosed acetylated low-density lipoproteins. They are also characterized by the presence of intracellular Weibel-Palade bodies and caveolae and microvillous projections on the luminal surface.
The NhGEC will be evaluated for the expression of endothelial specific markers (e.g. CD31, vWF and Ve-Cadherin) by Immunohistochemistry and Western Blot.
In addition, assessment of cellular morphology will be performed by comparing them to a known standard.
NhGEC can be expanded between 5 to 8 passages, while maintaining their particular characteristics.
NhGEC are terminally differentiated cells and present no significant differentiation capacity.
The NhGEC are for research use only and not approved for human or animal use, nor for in vitro diagnostic testing.
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