Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Normal Human Kidney Proximal Tubule Cells- (NhKPT) are obtained from the cortical part of the human kidney and are an integral part of the nephron. The proximal tubule regulates the pH of the filtrate by exchanging hydrogen ions for bicarbonate ions in the filtrate; it is also responsible for secreting organic acids, such as creatinine and other bases, into the filtrate. The NhKPT cells are terminally differentiated and can be expanded for at least 15 population doublings.
NhKPT cells are isolated from the kidney cortex after removal of the kidney capsule and medulla. After digestion of the cortex, NhKPT are isolated by CD10 and CD13 positive selection as a pure population.
Expression of kidney proximal tubule specific markers like aquaporin 1, NHE3, and Na+K+ATPase are tested by immunostaining and qPCR. Absence of E-Cadherin and MUC1 (distal tubule markers) is assessed by immunofluorescence and qPCR.
The NhKPT cells are for research use only and not approved for human or animal use, nor for in vitro diagnostic testing.