Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage dewar. Cells are viable for at least 2 years when stored under these conditions.
Proliferating Normal Human Bronchial Epithelial Cells- P0 (NhBE-P0) are isolated from the bronchus at the bifurcation point to the fourth generation of bronchi. The cell composition consists of undifferentiated ciliated, non-ciliated, goblet, and basal cells.
Proliferating NhBE-P0 cells have been characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).
The NhBE-P0 cells are guaranteed to have minimum population doublings of at least 20 (from P0 to P2) following the instructions and conditions provided by Novabiosis.
Proliferating NhBE-P0 are for research use only and not approved for human or animal use, nor for in vitro diagnostic testing.