Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Proliferating Normal Human Small Airway Epithelial Cells- P1 (NhSAE-P1) are initially isolated as NhSAE-P0 cells from the fifth generation of bronchi down to small airways near 1mm in diameter. The NhSAE-P0 cells are then expanded on plastic using BEGM, CRC or Dual Smad expansion media to isolate out an homogenous basal cell population known as NhSAE-P1.
Proliferating NhSAE-P1 cells have been characterized by morphological assessment by light microscopy. The NhSAE-P1 homogenous basal cell population was then further QC tested after differentiating the NhSAE-P1 cells into ciliated, non-ciliated and goblet cells. These differentiated cells were characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).
The NhSAE-P1 cells are guaranteed to have minimum population doublings of at least 20 (from P1 to P3) following the instructions and conditions provided by Novabiosis.
Proliferating NhSAE-P1 are for research use only and not approved for human or animal use, nor for in vitro diagnostic testing.