Upon receipt, immediately transfer each cryovial into the vapor phase of a liquid nitrogen storage tank. Cells are viable for at least 2 years when stored under these conditions.
Proliferating Normal Human Small Airway Epithelial Cells- P1 (NhSAE-P1) are initially isolated as NhSAE-P0 cells from the fifth generation of bronchi down to small airways near 1mm in diameter. The NhSAE-P0 cells are then expanded on plastic using BEGM, CRC or Dual Smad expansion media to isolate out an homogenous basal cell population known as NhSAE-P1.
Proliferating NhSAE-P1 cells have been characterized by morphological assessment by light microscopy. The NhSAE-P1 homogenous basal cell population was then further QC tested after differentiating the NhSAE-P1 cells into ciliated, non-ciliated and goblet cells. These differentiated cells were characterized and Air/Liquid Interface culture (ALI) validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5).
The NhSAE-P1 cells are guaranteed to have minimum population doublings of at least 20 (from P1 to P3) following the instructions and conditions provided by Novabiosis.
THESE PRODUCTS ARE FOR RESEARCH USE ONLY and NOT approved for use in humans or clinical diagnostic testing.